› 4tt8

X-ray diffraction
2.3Å resolution

Crystal structure of the hydrolase domain of 10-formyltetrahydrofolate dehydrogenase (wild-type) complex with 10-formyl-5,8-dideazafolate

Released:
DOI: 10.2210/pdb4tt8/pdb
PDB entry 4tt8 coloured by chain, front view.
PDB entry 4tt8 coloured by chain, side view.
PDB entry 4tt8 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Structures of the hydrolase domain of zebrafish 10-formyltetrahydrofolate dehydrogenase and its complexes reveal a complete set of key residues for hydrolysis and product inhibition.
Lin CC, Chuankhayan P, Chang WN, Kao TT, Guan HH, Fun HK, Nakagawa A, Fu TF, Chen CJ
Acta Crystallogr D Biol Crystallogr 71 1006-21 (2015)
PMID: 25849409

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + NADP(+) + H(2)O = tetrahydrofolate + CO(2) + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
10-formyltetrahydrofolate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 35.46 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: E3NZ06 (Residues: 1-311; Coverage: 34%)
Gene name: aldh1l1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 6DD 1 x 6DD
Ligand BTB 1 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P21212
Unit cell:
a: 104.075Å b: 52.694Å c: 60.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.179 0.224
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

The Incoherent Fluctuation of Folate Pools and Differential Regulation of Folate Enzymes Prioritize Nucleotide Supply in the Zebrafish Model Displaying Folate Deficiency-Induced Microphthalmia and Visual Defects.
Hsiao et al. (2021)
2 more

3 mentions without citation

The Role of Single-Nucleotide Polymorphisms in the Function of Candidate Tumor Suppressor ALDH1L1.
Krupenko et al. (2019)
2 more