› 4tui

X-ray diffraction
3.59Å resolution

Crystal structure of MjMre11-DNA1 complex

Released:
DOI: 10.2210/pdb4tui/pdb
PDB entry 4tui coloured by chain, front view.
PDB entry 4tui coloured by chain, side view.
PDB entry 4tui coloured by chain, top view.
Source organisms:
Primary publication:
DNA end recognition by the Mre11 nuclease dimer: insights into resection and repair of damaged DNA.
Sung S, Li F, Park YB, Kim JS, Kim AK, Song OK, Kim J, Che J, Lee SE, Cho Y
EMBO J 33 2422-35 (2014)
PMID: 25107472

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118168 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA double-strand break repair protein Mre11 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 337 amino acids
Theoretical weight: 39.7 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58719 (Residues: 1-333; Coverage: 91%)
Gene names: MJ1323, mre11
Sequence domains: Calcineurin-like phosphoesterase
Structure domains:
DNA (5'-D(P*TP*GP*GP*CP*AP*CP*GP*TP*AP*GP*GP*AP*C)-3') Chain: G
Molecule details ›
Chain: G
Length: 13 nucleotides
Theoretical weight: 4.02 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*CP*CP*TP*AP*CP*GP*TP*GP*CP*CP*AP*G)-3') Chain: H
Molecule details ›
Chain: H
Length: 13 nucleotides
Theoretical weight: 3.93 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 90.468Å b: 183.966Å c: 106.538Å
α: 90° β: 99.46° γ: 90°
R-values:
R R work R free
0.219 0.217 0.259
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

The MRE11-RAD50-NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair.
Syed et al. (2018)
10 more

1 mention without citation

DNA end recognition by the Mre11 nuclease dimer: insights into resection and repair of damaged DNA.
Sung et al. (2014)