› 4tv0

X-ray diffraction
2.6Å resolution

Drosophila stem-loop binding protein complexed with histone mRNA stem-loop, Selenomethionine derivative

Released:
DOI: 10.2210/pdb4tv0/pdb
PDB entry 4tv0 coloured by chain, front view.
PDB entry 4tv0 coloured by chain, side view.
PDB entry 4tv0 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular mechanisms for the regulation of histone mRNA stem-loop-binding protein by phosphorylation.
Zhang J, Tan D, DeRose EF, Perera L, Dominski Z, Marzluff WF, Tong L, Hall TM
Proc Natl Acad Sci U S A 111 E2937-46 (2014)
PMID: 25002523

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194207 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Histone RNA hairpin-binding protein Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 11.01 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VAN6 (Residues: 184-267; Coverage: 30%)
Gene names: CG11886, Slbp
Sequence domains: Histone RNA hairpin-binding protein RNA-binding domain
RNA (26-MER) Chain: B
Molecule details ›
Chain: B
Length: 26 nucleotides
Theoretical weight: 8.29 KDa
Source organism: synthetic construct
Expression system: Not provided
Sequence domains: Histone 3' UTR stem-loop

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P4132
Unit cell:
a: 103.929Å b: 103.929Å c: 103.929Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.25 0.284
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Birth and Death of Histone mRNAs.
Marzluff et al. (2017)
3 more

4 mentions without citation

RNA Structures as Mediators of Neurological Diseases and as Drug Targets.
Bernat et al. (2015)
3 more