› 4tvd

X-ray diffraction
2.3Å resolution

N-terminally truncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with D-glucose

Released:
DOI: 10.2210/pdb4tvd/pdb
PDB entry 4tvd coloured by chain, front view.
PDB entry 4tvd coloured by chain, side view.
PDB entry 4tvd coloured by chain, top view.
Source organism: Leuconostoc mesenteroides subsp. mesenteroides
Primary publication:
Structural Insights into the Carbohydrate Binding Ability of an α-(1→2) Branching Sucrase from Glycoside Hydrolase Family 70.
Brison Y, Malbert Y, Czaplicki G, Mourey L, Remaud-Simeon M, Tranier S
J Biol Chem 291 7527-40 (2016)
PMID: 26865636

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124649 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dextransucrase Chain: A
Molecule details ›
Chain: A
Length: 1108 amino acids
Theoretical weight: 124.1 KDa
Source organism: Leuconostoc mesenteroides subsp. mesenteroides
Expression system: Escherichia coli
UniProt:
  • Canonical: G8XR50 (Residues: 1760-2836; Coverage: 39%)
Gene name: dsrE563
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 1 x CA
Ligand BGC 9 x BGC
Ligand GLC 1 x GLC
Ligand PEG 1 x PEG
2 modified residues:
Ligand TYI 1 x TYI
Ligand IYR 3 x IYR

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 67.77Å b: 98.98Å c: 181.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.169 0.223
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Bacterial α-Glucan and Branching Sucrases from GH70 Family: Discovery, Structure-Function Relationship Studies and Engineering.
Molina et al. (2021)
6 other articles

3 mentions without citation

Immobilization of Glycoside Hydrolase Families GH1, GH13, and GH70: State of the Art and Perspectives.
Graebin et al. (2016)
2 more