› 4tvm

X-ray diffraction
2.6Å resolution

Structure of Citrate Synthase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb4tvm/pdb
PDB entry 4tvm coloured by chain, front view.
PDB entry 4tvm coloured by chain, side view.
PDB entry 4tvm coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structures of citrate synthase and malate dehydrogenase of Mycobacterium tuberculosis.
Ferraris DM, Spallek R, Oehlmann W, Singh M, Rizzi M
Proteins 83 389-94 (2015)
PMID: 25524525

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161968 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate synthase 1 Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 48 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPD5 (Residues: 1-431; Coverage: 100%)
Gene names: MTCY31.24, Rv0896, gltA, gltA2
Sequence domains: Citrate synthase, C-terminal domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand OAA 1 x OAA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: R32
Unit cell:
a: 148.767Å b: 148.767Å c: 182.797Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.174 0.194
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Mycobacterium tuberculosis Molecular Determinants of Infection, Survival Strategies, and Vulnerable Targets.
Ferraris et al. (2018)
9 other articles