› 4tx8

X-ray diffraction
2.17Å resolution

Crystal Structure of a Family GH18 Chitinase from Chromobacterium violaceum

Released:
DOI: 10.2210/pdb4tx8/pdb
PDB entry 4tx8 coloured by chain, front view.
PDB entry 4tx8 coloured by chain, side view.
PDB entry 4tx8 coloured by chain, top view.
Source organism: Chromobacterium violaceum ATCC 12472
Entry authors: Pereira HM, Lobo MDP, Brandao-Neto J, Grangeiro TB

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-181831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
chitinase Chain: A
Molecule details ›
Chain: A
Length: 439 amino acids
Theoretical weight: 45.75 KDa
Source organism: Chromobacterium violaceum ATCC 12472
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q7NTW8 (Residues: 1-439; Coverage: 100%)
Gene name: CV_2935
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NAG 1 x NAG
Ligand MG 1 x MG
Ligand CA 1 x CA
Ligand FLC 4 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P43212
Unit cell:
a: 120.48Å b: 120.48Å c: 106.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.191
Expression system: Komagataella pastoris

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