› 4txd

X-ray diffraction
1.82Å resolution

Crystal structure of Thermofilum pendens Csc2

Released:
DOI: 10.2210/pdb4txd/pdb
PDB entry 4txd coloured by chain, front view.
PDB entry 4txd coloured by chain, side view.
PDB entry 4txd coloured by chain, top view.
Source organism: Thermofilum pendens
Primary publication:
Structural analyses of the CRISPR protein Csc2 reveal the RNA-binding interface of the type I-D Cas7 family.
Hrle A, Maier LK, Sharma K, Ebert J, Basquin C, Urlaub H, Marchfelder A, Conti E
RNA Biol 11 1072-82 (2014)
PMID: 25483036

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type I-D CRISPR-associated protein Cas7/Csc2 Chain: A
Molecule details ›
Chain: A
Length: 390 amino acids
Theoretical weight: 43.4 KDa
Source organism: Thermofilum pendens
Expression system: Escherichia coli
UniProt:
  • Canonical: A1RZU2 (Residues: 1-374; Coverage: 100%)
Gene name: Tpen_1325
Sequence domains: Csc2 Crispr

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 80.951Å b: 112.591Å c: 60.465Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.212
Expression system: Escherichia coli

Quick links

Citations

4 review citations

DNA and RNA interference mechanisms by CRISPR-Cas surveillance complexes.
Plagens et al. (2015)
3 more

3 mentions without citation

Structure Principles of CRISPR-Cas Surveillance and Effector Complexes.
Tsui et al. (2015)
2 more