› 4u0n

X-ray diffraction
2.1Å resolution

Structure of the Vibrio cholerae di-nucleotide cyclase (DncV) deletion mutant D-loop

Released:
DOI: 10.2210/pdb4u0n/pdb
PDB entry 4u0n coloured by chain, front view.
PDB entry 4u0n coloured by chain, side view.
PDB entry 4u0n coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Primary publication:
Structural biochemistry of a Vibrio cholerae dinucleotide cyclase reveals cyclase activity regulation by folates.
Zhu D, Wang L, Shang G, Liu X, Zhu J, Lu D, Wang L, Kan B, Zhang JR, Xiang Y
Mol Cell 55 931-937 (2014)
PMID: 25201413

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 44.74 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KVG7 (Residues: 1-419; Coverage: 88%)
Gene names: VC_0179, dncV
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand TLL 2 x TLL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 73.605Å b: 49.253Å c: 122.233Å
α: 90° β: 98.66° γ: 90°
R-values:
R R work R free
0.22 0.218 0.264
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA.
Kato et al. (2017)
14 more

1 mention without citation

FAM46 proteins are novel eukaryotic non-canonical poly(A) polymerases.
Kuchta et al. (2016)