› 4u14

X-ray diffraction
3.57Å resolution

Structure of the M3 muscarinic acetylcholine receptor bound to the antagonist tiotropium crystallized with disulfide-stabilized T4 lysozyme (dsT4L)

Released:
DOI: 10.2210/pdb4u14/pdb
PDB entry 4u14 coloured by chain, front view.
PDB entry 4u14 coloured by chain, side view.
PDB entry 4u14 coloured by chain, top view.
Source organisms:
Primary publication:
Modified T4 Lysozyme Fusion Proteins Facilitate G Protein-Coupled Receptor Crystallogenesis.
Thorsen TS, Matt R, Weis WI, Kobilka BK
Structure 22 1657-64 (2014)
PMID: 25450769

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132957 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Muscarinic acetylcholine receptor M3 Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 52.68 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08483 (Residues: 57-259, 482-563; Coverage: 48%)
  • Canonical: P00720 (Residues: 1-161; Coverage: 98%)
Gene names: Chrm-3, Chrm3, E
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand 0HK 1 x 0HK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P41212
Unit cell:
a: 54.952Å b: 54.952Å c: 348.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.275 0.272 0.325
Expression system: Spodoptera frugiperda

Quick links

Citations

13 review citations

Atomic-level analysis of membrane-protein structure.
Hendrickson WA. (2016)
12 more

25 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
24 more