› 4u15

X-ray diffraction
2.8Å resolution

M3-mT4L receptor bound to tiotropium

Released:
DOI: 10.2210/pdb4u15/pdb
PDB entry 4u15 coloured by chain, front view.
PDB entry 4u15 coloured by chain, side view.
PDB entry 4u15 coloured by chain, top view.
Source organisms:
Primary publication:
Modified T4 Lysozyme Fusion Proteins Facilitate G Protein-Coupled Receptor Crystallogenesis.
Thorsen TS, Matt R, Weis WI, Kobilka BK
Structure 22 1657-64 (2014)
PMID: 25450769

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Muscarinic acetylcholine receptor M3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 418 amino acids
Theoretical weight: 47.86 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08483 (Residues: 57-259, 482-563; Coverage: 48%)
  • Canonical: D9IEF7 (Residues: 61-161; Coverage: 62%)
Gene names: Chrm-3, Chrm3, T4Tp126, e
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand 0HK 2 x 0HK
Ligand P6G 4 x P6G
Ligand TAR 4 x TAR
Ligand OLC 2 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 152.311Å b: 184.563Å c: 52.613Å
α: 90° β: 98.54° γ: 90°
R-values:
R R work R free
0.232 0.23 0.261
Expression system: Spodoptera frugiperda

Quick links

Citations

12 review citations

Atomic-level analysis of membrane-protein structure.
Hendrickson WA. (2016)
11 more

41 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
40 more