› 4u1d

X-ray diffraction
3.3Å resolution

Structure of the PCI domain of translation initiation factor eIF3a

Released:
DOI: 10.2210/pdb4u1d/pdb
PDB entry 4u1d coloured by chain, front view.
PDB entry 4u1d coloured by chain, side view.
PDB entry 4u1d coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Molecular architecture of the 40S⋅eIF1⋅eIF3 translation initiation complex.
Erzberger JP, Stengel F, Pellarin R, Zhang S, Schaefer T, Aylett CHS, Cimermančič P, Boehringer D, Sali A, Aebersold R, Ban N
Cell 158 1123-1135 (2014)
PMID: 25171412

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153591 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 3 subunit A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 497 amino acids
Theoretical weight: 57.01 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38249 (Residues: 1-494; Coverage: 51%)
Gene names: RPG1, TIF32, YBR0734, YBR079C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 242.53Å b: 158.27Å c: 93.74Å
α: 90° β: 104.21° γ: 90°
R-values:
R R work R free
0.172 0.17 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

41 review citations

The nuclear pore complex: understanding its function through structural insight.
Beck et al. (2017)
40 more