› 4u2j

X-ray diffraction
2Å resolution

N-terminal domain of C. Reinhardtii SAS-6 homolog bld12p variant Q93E F145W (NN27)

Released:
DOI: 10.2210/pdb4u2j/pdb
PDB entry 4u2j coloured by chain, front view.
PDB entry 4u2j coloured by chain, side view.
PDB entry 4u2j coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture.
Hilbert M, Noga A, Frey D, Hamel V, Guichard P, Kraatz SH, Pfreundschuh M, Hosner S, Flückiger I, Jaussi R, Wieser MM, Thieltges KM, Deupi X, Müller DJ, Kammerer RA, Gönczy P, Hirono M, Steinmetz MO
Nat Cell Biol 18 393-403 (2016)
PMID: 26999736

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108965 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spindle assembly abnormal protein 6 N-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 161 amino acids
Theoretical weight: 18.14 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A9CQL4 (Residues: 1-159; Coverage: 22%)
Gene names: CHLRE_12g516950v5, CrSAS-6
Sequence domains: Centriolar protein SAS N-terminal

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 40.15Å b: 97Å c: 142.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Once and only once: mechanisms of centriole duplication and their deregulation in disease.
Nigg et al. (2018)
11 more