› 4u35

X-ray diffraction
1.55Å resolution

Crystal Structures of RNA Duplexes Containing 2-thio-Uridine

Released:
DOI: 10.2210/pdb4u35/pdb
PDB entry 4u35 coloured by chain, front view.
PDB entry 4u35 coloured by chain, side view.
PDB entry 4u35 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Crystal structure studies of RNA duplexes containing s(2)U:A and s(2)U:U base pairs.
Sheng J, Larsen A, Heuberger BD, Blain JC, Szostak JW
J Am Chem Soc 136 13916-24 (2014)
PMID: 25188906

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196879 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (5'-R(*UP*AP*GP*CP*(SUR)P*CP*C-3') Chain: A
Molecule details ›
Chain: A
Length: 7 nucleotides
Theoretical weight: 2.17 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(*GP*GP*UP*GP*CP*UP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 7 nucleotides
Theoretical weight: 2.24 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SUR 1 x SUR

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 21.681Å b: 35.158Å c: 47.304Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.211
Expression system: Not provided

Quick links

Citations

5 review citations

The Importance of Being Modified: The Role of RNA Modifications in Translational Fidelity.
Agris et al. (2017)
4 more

2 mentions without citation

Thermodynamic insights into 2-thiouridine-enhanced RNA hybridization.
Larsen et al. (2015)
1 more