› 4u4t

X-ray diffraction
2.4Å resolution

Structure of a nitrate/nitrite antiporter NarK in nitrate-bound inward-open state

Released:
DOI: 10.2210/pdb4u4t/pdb
PDB entry 4u4t coloured by chain, front view.
PDB entry 4u4t coloured by chain, side view.
PDB entry 4u4t coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. MG1655
Primary publication:
Structural basis for dynamic mechanism of nitrate/nitrite antiport by NarK.
Fukuda M, Takeda H, Kato HE, Doki S, Ito K, Maturana AD, Ishitani R, Nureki O
Nat Commun 6 7097 (2015)
PMID: 25959928

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrate/nitrite antiporter NarK Chain: A
Molecule details ›
Chain: A
Length: 475 amino acids
Theoretical weight: 51.05 KDa
Source organism: Escherichia coli str. K-12 substr. MG1655
Expression system: Escherichia coli
UniProt:
  • Canonical: P10903 (Residues: 1-463; Coverage: 100%)
Gene names: JW1214, b1223, narK
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

4 bound ligands:
Ligand NO3 1 x NO3
Ligand ZN 1 x ZN
Ligand OLA 2 x OLA
Ligand OLC 5 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P212121
Unit cell:
a: 35.394Å b: 109.358Å c: 126.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.217 0.248
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
5 more

3 mentions without citation

Structural basis for dynamic mechanism of nitrate/nitrite antiport by NarK.
Fukuda et al. (2015)
2 more