› 4u64

X-ray diffraction
2.14Å resolution

Structure of the periplasmic output domain of the Legionella pneumophila LapD ortholog CdgS9 in the apo state

Released:
DOI: 10.2210/pdb4u64/pdb
PDB entry 4u64 coloured by chain, front view.
PDB entry 4u64 coloured by chain, side view.
PDB entry 4u64 coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Primary publication:
Mechanistic insight into the conserved allosteric regulation of periplasmic proteolysis by the signaling molecule cyclic-di-GMP.
Chatterjee D, Cooley RB, Boyd CD, Mehl RA, O'Toole GA, Sondermann H
Elife 3 e03650 (2014)
PMID: 25182848

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Two component histidine kinase, GGDEF domain protein/EAL domain protein Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 15.12 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZXA3 (Residues: 22-152; Coverage: 21%)
Gene name: lpg0829
Sequence domains: LapD/MoxY periplasmic domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P6122
Unit cell:
a: 61.581Å b: 61.581Å c: 147.528Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.219 0.243
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Cyclic di-GMP: second messenger extraordinaire.
Jenal et al. (2017)
13 more

5 mentions without citation

Contribution of Physical Interactions to Signaling Specificity between a Diguanylate Cyclase and Its Effector.
Dahlstrom et al. (2015)
4 more