› 4u7j

X-ray diffraction
1.75Å resolution

Crystal structure of Argininosuccinate synthase from Mycobacterium thermoresistibile

Released:
DOI: 10.2210/pdb4u7j/pdb
PDB entry 4u7j coloured by chain, front view.
PDB entry 4u7j coloured by chain, side view.
PDB entry 4u7j coloured by chain, top view.
Source organism: Mycolicibacterium thermoresistibile ATCC 19527
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Argininosuccinate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 408 amino acids
Theoretical weight: 45.18 KDa
Source organism: Mycolicibacterium thermoresistibile ATCC 19527
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G7CBN9 (Residues: 1-400; Coverage: 100%)
Gene names: KEK_01915, argG
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand EDO 13 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21212
Unit cell:
a: 93.94Å b: 144.44Å c: 57.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.182
Expression system: Escherichia coli BL21(DE3)

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