› 4u7u

X-ray diffraction
3Å resolution

Crystal structure of RNA-guided immune Cascade complex from E.coli

Released:
DOI: 10.2210/pdb4u7u/pdb
PDB entry 4u7u coloured by chain, front view.
PDB entry 4u7u coloured by chain, side view.
PDB entry 4u7u coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli.
Zhao H, Sheng G, Wang J, Wang M, Bunkoczi G, Gong W, Wei Z, Wang Y
Nature 515 147-50 (2014)
PMID: 25118175

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero 24-mer
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160115 (preferred)
Entry contents:
5 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (6 distinct):
CRISPR system Cascade subunit CasA Chains: A, M
Molecule details ›
Chains: A, M
Length: 502 amino acids
Theoretical weight: 55.96 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46901 (Residues: 1-502; Coverage: 100%)
Gene names: JW2730, b2760, casA, cse1, ygcL
Sequence domains: CRISPR-associated protein Cse1 (CRISPR_cse1)
CRISPR system Cascade subunit CasB Chains: B, C, N, O
Molecule details ›
Chains: B, C, N, O
Length: 160 amino acids
Theoretical weight: 18.73 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P76632 (Residues: 1-160; Coverage: 100%)
Gene names: JW2729, b2759, casB, cse2, ygcK
Sequence domains: CRISPR-associated protein Cse2 (CRISPR_cse2)
Structure domains: CRISPR-associated protein Cse2
CRISPR system Cascade subunit CasE Chains: D, P
Molecule details ›
Chains: D, P
Length: 201 amino acids
Theoretical weight: 22.58 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46897 (Residues: 1-199; Coverage: 100%)
Gene names: JW2726, b2756, cas6e, casE, ygcH
Sequence domains: CRISPR associated protein
Structure domains:
CRISPR system Cascade subunit CasC Chains: E, F, G, H, I, J, Q, R, S, T, U, V
Molecule details ›
Chains: E, F, G, H, I, J, Q, R, S, T, U, V
Length: 363 amino acids
Theoretical weight: 40.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46899 (Residues: 1-363; Coverage: 100%)
Gene names: JW2728, b2758, cas4, casC, cse4, ygcJ
Sequence domains: CT1975-like protein
CRISPR system Cascade subunit CasD Chains: K, W
Molecule details ›
Chains: K, W
Length: 224 amino acids
Theoretical weight: 25.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46898 (Residues: 1-224; Coverage: 100%)
Gene names: JW5844, b2757, cas5, casD, ygcI
Sequence domains: CRISPR-associated protein (Cas_Cas5)
Structure domains: Alpha-Beta Plaits
crRNA Chains: L, X
Molecule details ›
Chains: L, X
Length: 61 nucleotides
Theoretical weight: 19.63 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P1
Unit cell:
a: 111.412Å b: 118.143Å c: 225.873Å
α: 92.23° β: 93.55° γ: 106.06°
R-values:
R R work R free
0.182 0.18 0.218
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

27 review citations

An updated evolutionary classification of CRISPR-Cas systems.
Makarova et al. (2015)
26 more

5 mentions without citation

The structural biology of CRISPR-Cas systems.
Jiang et al. (2015)
4 more