› 4u9d

X-ray diffraction
2.5Å resolution

Crystal Structure of the Zn-directed tetramer of the engineered cyt cb562 variant, AB3

Released:
DOI: 10.2210/pdb4u9d/pdb
PDB entry 4u9d coloured by chain, front view.
PDB entry 4u9d coloured by chain, side view.
PDB entry 4u9d coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A designed supramolecular protein assembly with in vivo enzymatic activity.
Song WJ, Tezcan FA
Science 346 1525-8 (2014)
PMID: 25525249

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 106 amino acids
Theoretical weight: 11.84 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562
Structure domains: Cytochrome c/b562

Ligands and Environments

2 bound ligands:
Ligand HEC 4 x HEC
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P61
Unit cell:
a: 54.83Å b: 54.83Å c: 237.82Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.238 0.296
Expression system: Escherichia coli BL21(DE3)

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Citations

36 review citations

Methods for the directed evolution of proteins.
Packer et al. (2015)
35 more