› 4uat

X-ray diffraction
1.3Å resolution

Crystal structure of CbbY (mutant D10N) from Rhodobacter sphaeroides in complex with Xylulose-(1,5)bisphosphate, crystal form I

Released:
DOI: 10.2210/pdb4uat/pdb
PDB entry 4uat coloured by chain, front view.
PDB entry 4uat coloured by chain, side view.
PDB entry 4uat coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Degradation of potent Rubisco inhibitor by selective sugar phosphatase.
Bracher A, Sharma A, Starling-Windhof A, Hartl FU, Hayer-Hartl M
Nat Plants 1 14002 (2015)
PMID: 27246049

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161171 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein CbbY Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 25.15 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P95649 (Residues: 1-230; Coverage: 100%)
Gene name: cbbY
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand XBP 2 x XBP
Ligand MG 2 x MG
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 35.419Å b: 51.468Å c: 122.083Å
α: 90° β: 94.06° γ: 90°
R-values:
R R work R free
0.124 0.121 0.168
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Biogenesis and Metabolic Maintenance of Rubisco.
Bracher et al. (2017)
5 more

2 mentions without citation

From chaperonins to Rubisco assembly and metabolic repair.
Hayer-Hartl M. (2017)
1 more