› 4udu

X-ray diffraction
2.5Å resolution

Crystal structure of staphylococcal enterotoxin E in complex with a T cell receptor

Released:
DOI: 10.2210/pdb4udu/pdb
PDB entry 4udu coloured by chain, front view.
PDB entry 4udu coloured by chain, side view.
PDB entry 4udu coloured by chain, top view.
Source organisms:
Primary publication:
Structure of Staphylococcal Enterotoxin E in Complex with TCR Defines the Role of TCR Loop Positioning in Superantigen Recognition.
Rödström KE, Regenthal P, Lindkvist-Petersson K
PLoS One 10 e0131988 (2015)
PMID: 26147596

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-100939 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
T cell receptor alpha chain constant; T cell receptor alpha variable 22 Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 22.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0B4J277 (Residues: 21-110; Coverage: 100%)
  • Canonical: P01848 (Residues: 1-93; Coverage: 66%)
Gene names: TCRA, TRAC, TRAV22
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein; T cell receptor beta constant 2 Chain: B
Molecule details ›
Chain: B
Length: 243 amino acids
Theoretical weight: 27.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A5A3 (Residues: 20-115; Coverage: 99%)
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
Gene names: TCRBC2, TCRBV6S4A1, TRBC2
Sequence domains:
Structure domains: Immunoglobulins
Enterotoxin type E Chain: C
Molecule details ›
Chain: C
Length: 233 amino acids
Theoretical weight: 26.81 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P12993 (Residues: 25-257; Coverage: 100%)
Gene name: entE
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 63.134Å b: 78.544Å c: 180.805Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.246 0.255
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli K-12

Quick links

Citations

2 review citations

Staphylococcal enterotoxins and enterotoxin-like toxins with special reference to dairy products: An overview.
Benkerroum N. (2018)
1 more

2 mentions without citation

Structure of Staphylococcal Enterotoxin E in Complex with TCR Defines the Role of TCR Loop Positioning in Superantigen Recognition.
Rödström et al. (2015)
1 more