› 4uex

X-ray diffraction
1.8Å resolution

Structure of human Saposin A at lysosomal pH

Released:
DOI: 10.2210/pdb4uex/pdb
PDB entry 4uex coloured by chain, front view.
PDB entry 4uex coloured by chain, side view.
PDB entry 4uex coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human saposin A at lysosomal pH.
Hill CH, Read RJ, Deane JE
Acta Crystallogr F Struct Biol Commun 71 895-900 (2015)
PMID: 26144235

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139656 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Saposin-A Chains: A, B
Molecule details ›
Chains: A, B
Length: 85 amino acids
Theoretical weight: 9.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07602 (Residues: 60-142; Coverage: 16%)
Gene names: GLBA, PSAP, SAP1
Sequence domains:
Structure domains: Saposin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 34.17Å b: 58.69Å c: 35.16Å
α: 90° β: 93.1° γ: 90°
R-values:
R R work R free
0.186 0.185 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

The mechanism of glycosphingolipid degradation revealed by a GALC-SapA complex structure.
Hill et al. (2018)
3 more

2 mentions without citation

Structure of human saposin A at lysosomal pH.
Hill et al. (2015)
1 more