› 4ufa

X-ray diffraction
1.8Å resolution

Crystal structure of the Angiotensin-1 converting enzyme N-domain in complex with Ac-SD

Released:
DOI: 10.2210/pdb4ufa/pdb
PDB entry 4ufa coloured by chain, front view.
PDB entry 4ufa coloured by chain, side view.
PDB entry 4ufa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of Ac-SDKP hydrolysis by Angiotensin-I converting enzyme.
Masuyer G, Douglas RG, Sturrock ED, Acharya KR
Sci Rep 5 13742 (2015)
PMID: 26403559

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Angiotensin-converting enzyme, soluble form Chains: A, B
Molecule details ›
Chains: A, B
Length: 629 amino acids
Theoretical weight: 72.61 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P12821 (Residues: 30-657; Coverage: 49%)
Gene names: ACE, DCP, DCP1
Sequence domains: Angiotensin-converting enzyme

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
7 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand NAG 2 x NAG
Ligand PEG 4 x PEG
Ligand P6G 3 x P6G
Ligand SAC 2 x SAC
Ligand ASP 2 x ASP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 72.87Å b: 76.72Å c: 82.8Å
α: 88.65° β: 64.31° γ: 75.22°
R-values:
R R work R free
0.204 0.202 0.228
Expression system: Cricetulus griseus

Quick links

Citations

3 review citations

ACE2 and ACE: structure-based insights into mechanism, regulation and receptor recognition by SARS-CoV.
Lubbe et al. (2020)
2 more

3 mentions without citation

Upgraded AMBER Force Field for Zinc-Binding Residues and Ligands for Predicting Structural Properties and Binding Affinities in Zinc-Proteins.
Macchiagodena et al. (2020)
2 more