› 4ufb

X-ray diffraction
1.8Å resolution

Crystal structure of the Angiotensin-1 converting enzyme N-domain in complex with Lys-Pro

Released:
DOI: 10.2210/pdb4ufb/pdb
PDB entry 4ufb coloured by chain, front view.
PDB entry 4ufb coloured by chain, side view.
PDB entry 4ufb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of Ac-SDKP hydrolysis by Angiotensin-I converting enzyme.
Masuyer G, Douglas RG, Sturrock ED, Acharya KR
Sci Rep 5 13742 (2015)
PMID: 26403559

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Angiotensin-converting enzyme, soluble form Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 629 amino acids
Theoretical weight: 72.61 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P12821 (Residues: 30-657; Coverage: 49%)
Gene names: ACE, DCP, DCP1
Sequence domains: Angiotensin-converting enzyme

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC, BMA
Carbohydrate polymer
6 bound ligands:
Ligand LYS 4 x LYS
Ligand PRO 4 x PRO
Ligand ZN 4 x ZN
Ligand CL 4 x CL
Ligand PEG 10 x PEG
Ligand P6G 6 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 73.48Å b: 101.59Å c: 114.51Å
α: 85.46° β: 85.9° γ: 81.62°
R-values:
R R work R free
0.208 0.207 0.235
Expression system: Cricetulus griseus

Quick links

Citations

3 review citations

ACE2 and ACE: structure-based insights into mechanism, regulation and receptor recognition by SARS-CoV.
Lubbe et al. (2020)
2 more

1 mention without citation

Structural basis of Ac-SDKP hydrolysis by Angiotensin-I converting enzyme.
Masuyer et al. (2015)