› 4ufj

X-ray diffraction
2.2Å resolution

Mouse Galactocerebrosidase complexed with iso-galacto-fagomine lactam IGL

Released:
DOI: 10.2210/pdb4ufj/pdb
PDB entry 4ufj coloured by chain, front view.
PDB entry 4ufj coloured by chain, side view.
PDB entry 4ufj coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Azasugar inhibitors as pharmacological chaperones for Krabbe disease.
Hill CH, Viuff AH, Spratley SJ, Salamone S, Christensen SH, Read RJ, Moriarty NW, Jensen HH, Deane JE
Chem Sci 6 3075-3086 (2015)
PMID: 26029356

Function and Biology Details

Reaction catalysed: 
D-galactosyl-N-acylsphingosine + H(2)O = D-galactose + N-acylsphingosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157108 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Galactocerebrosidase Chain: A
Molecule details ›
Chain: A
Length: 654 amino acids
Theoretical weight: 74.6 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P54818 (Residues: 41-684; Coverage: 100%)
Gene name: Galc
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand IF7 1 x IF7
Ligand NAG 1 x NAG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: R32
Unit cell:
a: 249.85Å b: 249.85Å c: 77.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.165 0.198
Expression system: Homo sapiens

Quick links

Citations

13 review citations

Transition Metal Complexes and Photodynamic Therapy from a Tumor-Centered Approach: Challenges, Opportunities, and Highlights from the Development of TLD1433.
Monro et al. (2019)
12 more

1 mention without citation

Azasugar inhibitors as pharmacological chaperones for Krabbe disease.
Hill et al. (2015)