4ujc

Electron Microscopy
9.5Å resolution

mammalian 80S HCV-IRES initiation complex with eIF5B POST-like state

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 83-mer (preferred)
PDBe Complex ID:
PDB-CPX-124219 (preferred)
Entry contents:
77 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (83 distinct):
Eukaryotic translation initiation factor 5B Chain: AB
Molecule details ›
Chain: AB
Length: 627 amino acids
Theoretical weight: 70.79 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TRL5 (Residues: 638-1264; Coverage: 50%)
Gene name: EIF5B
Sequence domains:
60S RIBOSOMAL PROTEIN L8 Chain: BA
Molecule details ›
Chain: BA
Length: 257 amino acids
Theoretical weight: 28.09 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L3 Chain: BB
Molecule details ›
Chain: BB
Length: 403 amino acids
Theoretical weight: 46.21 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L4 Chain: BC
Molecule details ›
Chain: BC
Length: 427 amino acids
Theoretical weight: 47.8 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L5 Chain: BD
Molecule details ›
Chain: BD
Length: 297 amino acids
Theoretical weight: 34.43 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L6 Chain: BE
Molecule details ›
Chain: BE
Length: 158 amino acids
Theoretical weight: 18.22 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L7 Chain: BF
Molecule details ›
Chain: BF
Length: 248 amino acids
Theoretical weight: 29.29 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L7A Chain: BG
Molecule details ›
Chain: BG
Length: 266 amino acids
Theoretical weight: 30.06 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L9 Chain: BH
Molecule details ›
Chain: BH
Length: 192 amino acids
Theoretical weight: 21.9 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L10 Chain: BI
Molecule details ›
Chain: BI
Length: 214 amino acids
Theoretical weight: 24.66 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L11 Chain: BJ
Molecule details ›
Chain: BJ
Length: 178 amino acids
Theoretical weight: 20.29 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L13 Chain: BL
Molecule details ›
Chain: BL
Length: 211 amino acids
Theoretical weight: 24.32 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L14 Chain: BM
Molecule details ›
Chain: BM
Length: 215 amino acids
Theoretical weight: 23.49 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L15 Chain: BN
Molecule details ›
Chain: BN
Length: 204 amino acids
Theoretical weight: 24.21 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L13A Chain: BO
Molecule details ›
Chain: BO
Length: 203 amino acids
Theoretical weight: 23.63 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L17 Chain: BP
Molecule details ›
Chain: BP
Length: 184 amino acids
Theoretical weight: 21.44 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L18 Chain: BQ
Molecule details ›
Chain: BQ
Length: 188 amino acids
Theoretical weight: 21.69 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L19 Chain: BR
Molecule details ›
Chain: BR
Length: 196 amino acids
Theoretical weight: 23.54 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L18A Chain: BS
Molecule details ›
Chain: BS
Length: 176 amino acids
Theoretical weight: 20.81 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L21 Chain: BT
Molecule details ›
Chain: BT
Length: 160 amino acids
Theoretical weight: 18.61 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L22 Chain: BU
Molecule details ›
Chain: BU
Length: 128 amino acids
Theoretical weight: 14.81 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L23 Chain: BV
Molecule details ›
Chain: BV
Length: 140 amino acids
Theoretical weight: 14.89 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L24 Chain: BW
Molecule details ›
Chain: BW
Length: 157 amino acids
Theoretical weight: 17.83 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L23A Chain: BX
Molecule details ›
Chain: BX
Length: 156 amino acids
Theoretical weight: 17.74 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L26 Chain: BY
Molecule details ›
Chain: BY
Length: 145 amino acids
Theoretical weight: 17.3 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L27 Chain: BZ
Molecule details ›
Chain: BZ
Length: 136 amino acids
Theoretical weight: 15.84 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L27A Chain: Ba
Molecule details ›
Chain: Ba
Length: 148 amino acids
Theoretical weight: 16.6 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L29 Chain: Bb
Molecule details ›
Chain: Bb
Length: 159 amino acids
Theoretical weight: 17.8 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L30 Chain: Bc
Molecule details ›
Chain: Bc
Length: 115 amino acids
Theoretical weight: 12.81 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L31 Chain: Bd
Molecule details ›
Chain: Bd
Length: 125 amino acids
Theoretical weight: 14.49 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L32 Chain: Be
Molecule details ›
Chain: Be
Length: 135 amino acids
Theoretical weight: 15.9 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L35A Chain: Bf
Molecule details ›
Chain: Bf
Length: 110 amino acids
Theoretical weight: 12.56 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L34 Chain: Bg
Molecule details ›
Chain: Bg
Length: 117 amino acids
Theoretical weight: 13.33 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL29 Chain: Bh
Molecule details ›
Chain: Bh
Length: 123 amino acids
Theoretical weight: 14.59 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L36 Chain: Bi
Molecule details ›
Chain: Bi
Length: 105 amino acids
Theoretical weight: 12.29 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L37 Chain: Bj
Molecule details ›
Chain: Bj
Length: 97 amino acids
Theoretical weight: 11.11 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L38 Chain: Bk
Molecule details ›
Chain: Bk
Length: 70 amino acids
Theoretical weight: 8.24 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L39 Chain: Bl
Molecule details ›
Chain: Bl
Length: 51 amino acids
Theoretical weight: 6.43 KDa
Source organism: Oryctolagus cuniculus
UBIQUITIN-60S RIBOSOMAL PROTEIN L40 Chain: Bm
Molecule details ›
Chain: Bm
Length: 128 amino acids
Theoretical weight: 14.76 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L41 Chain: Bn
Molecule details ›
Chain: Bn
Length: 25 amino acids
Theoretical weight: 3.47 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L36A Chain: Bo
Molecule details ›
Chain: Bo
Length: 106 amino acids
Theoretical weight: 12.48 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L37A Chain: Bp
Molecule details ›
Chain: Bp
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L28 Chain: Bt
Molecule details ›
Chain: Bt
Length: 137 amino acids
Theoretical weight: 15.78 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN L10A Chain: Bu
Molecule details ›
Chain: Bu
Length: 210 amino acids
Theoretical weight: 23.17 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US2 Chain: CA
Molecule details ›
Chain: CA
Length: 263 amino acids
Theoretical weight: 29.43 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES1 Chain: CB
Molecule details ›
Chain: CB
Length: 264 amino acids
Theoretical weight: 30 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US5 Chain: CC
Molecule details ›
Chain: CC
Length: 293 amino acids
Theoretical weight: 31.38 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US3 Chain: CD
Molecule details ›
Chain: CD
Length: 243 amino acids
Theoretical weight: 26.73 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES4 Chain: CE
Molecule details ›
Chain: CE
Length: 263 amino acids
Theoretical weight: 29.51 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US7 Chain: CF
Molecule details ›
Chain: CF
Length: 204 amino acids
Theoretical weight: 22.91 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES6 Chain: CG
Molecule details ›
Chain: CG
Length: 249 amino acids
Theoretical weight: 28.75 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES7 Chain: CH
Molecule details ›
Chain: CH
Length: 194 amino acids
Theoretical weight: 22.17 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES8 Chain: CI
Molecule details ›
Chain: CI
Length: 208 amino acids
Theoretical weight: 24.26 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US4 Chain: CJ
Molecule details ›
Chain: CJ
Length: 194 amino acids
Theoretical weight: 22.64 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES10 Chain: CK
Molecule details ›
Chain: CK
Length: 165 amino acids
Theoretical weight: 18.93 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US17 Chain: CL
Molecule details ›
Chain: CL
Length: 158 amino acids
Theoretical weight: 18.47 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES12 Chain: CM
Molecule details ›
Chain: CM
Length: 132 amino acids
Theoretical weight: 14.54 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US15 Chain: CN
Molecule details ›
Chain: CN
Length: 151 amino acids
Theoretical weight: 17.26 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US11 Chain: CO
Molecule details ›
Chain: CO
Length: 151 amino acids
Theoretical weight: 16.3 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US19 Chain: CP
Molecule details ›
Chain: CP
Length: 145 amino acids
Theoretical weight: 17.08 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US9 Chain: CQ
Molecule details ›
Chain: CQ
Length: 146 amino acids
Theoretical weight: 16.48 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES17 Chain: CR
Molecule details ›
Chain: CR
Length: 135 amino acids
Theoretical weight: 15.58 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US13 Chain: CS
Molecule details ›
Chain: CS
Length: 152 amino acids
Theoretical weight: 17.76 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES19 Chain: CT
Molecule details ›
Chain: CT
Length: 145 amino acids
Theoretical weight: 16.09 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US10 Chain: CU
Molecule details ›
Chain: CU
Length: 119 amino acids
Theoretical weight: 13.4 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES21 Chain: CV
Molecule details ›
Chain: CV
Length: 83 amino acids
Theoretical weight: 9.12 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US8 Chain: CW
Molecule details ›
Chain: CW
Length: 130 amino acids
Theoretical weight: 14.87 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US12 Chain: CX
Molecule details ›
Chain: CX
Length: 143 amino acids
Theoretical weight: 15.84 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES24 Chain: CY
Molecule details ›
Chain: CY
Length: 133 amino acids
Theoretical weight: 15.46 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES25 Chain: CZ
Molecule details ›
Chain: CZ
Length: 125 amino acids
Theoretical weight: 13.78 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES26 Chain: Ca
Molecule details ›
Chain: Ca
Length: 115 amino acids
Theoretical weight: 13.05 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES27 Chain: Cb
Molecule details ›
Chain: Cb
Length: 84 amino acids
Theoretical weight: 9.48 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES28 Chain: Cc
Molecule details ›
Chain: Cc
Length: 69 amino acids
Theoretical weight: 7.86 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN US14 Chain: Cd
Molecule details ›
Chain: Cd
Length: 56 amino acids
Theoretical weight: 6.69 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES30 Chain: Ce
Molecule details ›
Chain: Ce
Length: 59 amino acids
Theoretical weight: 6.67 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN ES31 Chain: Cf
Molecule details ›
Chain: Cf
Length: 156 amino acids
Theoretical weight: 18 KDa
Source organism: Oryctolagus cuniculus
40S RIBOSOMAL PROTEIN RACK1 Chain: Cg
Molecule details ›
Chain: Cg
Length: 317 amino acids
Theoretical weight: 35.12 KDa
Source organism: Oryctolagus cuniculus
tRNA Chain: AA
Molecule details ›
Chain: AA
Length: 76 nucleotides
Theoretical weight: 24.49 KDa
Sequence domains: tRNA
HCV-IRES Chain: AC
Molecule details ›
Chain: AC
Length: 504 nucleotides
Theoretical weight: 162.19 KDa
Sequence domains:
  • Hepatitis C virus internal ribosome entry site
  • Hepatitis C alternative reading frame stem-loop
  • 28S ribosomal RNA Chain: A2
    Molecule details ›
    Chain: A2
    Length: 5025 nucleotides
    Theoretical weight: 1625.92 KDa
    Sequence domains: Eukaryotic large subunit ribosomal RNA
    5.8S ribosomal RNA Chain: A3
    Molecule details ›
    Chain: A3
    Length: 194 nucleotides
    Theoretical weight: 62.62 KDa
    Sequence domains: 5.8S ribosomal RNA
    5S ribosomal RNA Chain: A4
    Molecule details ›
    Chain: A4
    Length: 121 nucleotides
    Theoretical weight: 39 KDa
    Sequence domains: 5S ribosomal RNA
    18S Ribosomal RNA Chain: C1
    Molecule details ›
    Chain: C1
    Length: 1869 nucleotides
    Theoretical weight: 602.78 KDa
    Sequence domains: Eukaryotic small subunit ribosomal RNA

    Ligands and Environments

    2 bound ligands:
    No modified residues

    Experiments and Validation Details

    Entry percentile scores
    Resolution: 9.5Å
    Relevant EMDB volumes: EMD-2683