› 4ulw

X-ray diffraction
1.91Å resolution

Crystal structure of the ROQ-domain of human ROQUIN1

Released:
DOI: 10.2210/pdb4ulw/pdb
PDB entry 4ulw coloured by chain, front view.
PDB entry 4ulw coloured by chain, side view.
PDB entry 4ulw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Roquin binding to target mRNAs involves a winged helix-turn-helix motif.
Schuetz A, Murakawa Y, Rosenbaum E, Landthaler M, Heinemann U
Nat Commun 5 5701 (2014)
PMID: 25504471

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178321 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Roquin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 152 amino acids
Theoretical weight: 17.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5TC82 (Residues: 177-328; Coverage: 13%)
Gene names: KIAA2025, RC3H1, RNF198
Sequence domains:
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 169.66Å b: 29.785Å c: 60.197Å
α: 90° β: 100.94° γ: 90°
R-values:
R R work R free
0.202 0.2 0.241
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Regulation of T cell signaling and autoimmunity by RNA-binding proteins.
Jeltsch et al. (2016)
5 more

1 mention without citation

New Insights into the RNA-Binding and E3 Ubiquitin Ligase Activities of Roquins.
Zhang et al. (2015)