› 4ums

X-ray diffraction
1.84Å resolution

The crystal structure of the seventh ScaB type I cohesin from Pseudobacteroides cellulosolvens

Released:
DOI: 10.2210/pdb4ums/pdb
PDB entry 4ums coloured by chain, front view.
PDB entry 4ums coloured by chain, side view.
PDB entry 4ums coloured by chain, top view.
Source organism: Pseudobacteroides cellulosolvens
Primary publication:
Combined Crystal Structure of a Type I Cohesin: MUTATION AND AFFINITY BINDING STUDIES REVEAL STRUCTURAL DETERMINANTS OF COHESIN-DOCKERIN SPECIFICITIES.
Cameron K, Weinstein JY, Zhivin O, Bule P, Fleishman SJ, Alves VD, Gilbert HJ, Ferreira LM, Fontes CM, Bayer EA, Najmudin S
J Biol Chem 290 16215-25 (2015)
PMID: 25934389

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179393 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SLH domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 156 amino acids
Theoretical weight: 16.96 KDa
Source organism: Pseudobacteroides cellulosolvens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6A564 (Residues: 1039-1186; Coverage: 6%)
Gene name: scaB
Sequence domains: Cohesin domain
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 30.382Å b: 52.767Å c: 90.457Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.256
Expression system: Escherichia coli BL21(DE3)

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Citations

5 citation in other articles

Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system.
Zhivin et al. (2017)
4 more

5 mentions without citation

Combining in Vitro and in Silico Single-Molecule Force Spectroscopy to Characterize and Tune Cellulosomal Scaffoldin Mechanics.
Verdorfer et al. (2017)
4 more