› 4umv

X-ray diffraction
3.2Å resolution

CRYSTAL STRUCTURE OF A ZINC-TRANSPORTING PIB-TYPE ATPASE IN THE E2P STATE

Released:
DOI: 10.2210/pdb4umv/pdb
PDB entry 4umv coloured by chain, front view.
PDB entry 4umv coloured by chain, side view.
PDB entry 4umv coloured by chain, top view.
Source organism: Shigella sonnei
Primary publication:
Structure and mechanism of Zn2+-transporting P-type ATPases.
Wang K, Sitsel O, Meloni G, Autzen HE, Andersson M, Klymchuk T, Nielsen AM, Rees DC, Nissen P, Gourdon P
Nature 514 518-22 (2014)
PMID: 25132545

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O + Zn(2+)(Side 1) = ADP + phosphate + Zn(2+)(Side 2)
ATP + H(2)O + Cd(2+)(Side 1) = ADP + phosphate + Cd(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174898 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Zinc/cadmium/lead-transporting P-type ATPase Chain: A
Molecule details ›
Chain: A
Length: 732 amino acids
Theoretical weight: 76.84 KDa
Source organism: Shigella sonnei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3YW59 (Residues: 1-732; Coverage: 100%)
Gene names: SSON_3707, zntA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand BEF 1 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 54.462Å b: 60.972Å c: 141.523Å
α: 90° β: 95.99° γ: 90°
R-values:
R R work R free
0.222 0.218 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Cation Diffusion Facilitator family: Structure and function.
Kolaj-Robin et al. (2015)
13 more

6 mentions without citation

Bacterial Strategies to Maintain Zinc Metallostasis at the Host-Pathogen Interface.
Capdevila et al. (2016)
5 more