› 4uno

X-ray diffraction
1.95Å resolution

Crystal structure of the ETS domain of human ETV5 in complex with DNA

Released:
DOI: 10.2210/pdb4uno/pdb
PDB entry 4uno coloured by chain, front view.
PDB entry 4uno coloured by chain, side view.
PDB entry 4uno coloured by chain, top view.
Source organisms:
Primary publication:
Structures of the Ets Protein DNA-binding Domains of Transcription Factors Etv1, Etv4, Etv5, and Fev: DETERMINANTS OF DNA BINDING AND REDOX REGULATION BY DISULFIDE BOND FORMATION.
Cooper CD, Newman JA, Aitkenhead H, Allerston CK, Gileadi O
J Biol Chem 290 13692-709 (2015)
PMID: 25866208

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118210 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
ETS translocation variant 5 Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41161 (Residues: 365-462; Coverage: 19%)
Gene names: ERM, ETV5
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*AP*CP*CP*GP*GP*AP*AP*GP*TP*GP)-3' Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*AP*CP*TP*TP*CP*CP*GP*GP*TP*CP)-3' Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3212
Unit cell:
a: 58.022Å b: 58.022Å c: 84.711Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.173 0.203
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Genetic-epigenetic interactions in cis: a major focus in the post-GWAS era.
Do et al. (2017)
2 more

5 mentions without citation

Structures of the Ets Protein DNA-binding Domains of Transcription Factors Etv1, Etv4, Etv5, and Fev: DETERMINANTS OF DNA BINDING AND REDOX REGULATION BY DISULFIDE BOND FORMATION.
Cooper et al. (2015)
4 more