› 4uo6

X-ray diffraction
2.9Å resolution

Structure of the A_Canine_Colorado_17864_06 H3 haemagglutinin in complex with Sialyl Lewis X

Released:
DOI: 10.2210/pdb4uo6/pdb
PDB entry 4uo6 coloured by chain, front view.
PDB entry 4uo6 coloured by chain, side view.
PDB entry 4uo6 coloured by chain, top view.
Source organism: Influenza A virus (A/canine/Colorado/17864/2006(H3N8))
Primary publication:
Recent evolution of equine influenza and the origin of canine influenza.
Collins PJ, Vachieri SG, Haire LF, Ogrodowicz RW, Martin SR, Walker PA, Xiong X, Gamblin SJ, Skehel JJ
Proc Natl Acad Sci U S A 111 11175-80 (2014)
PMID: 25024224

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122703 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (6 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 36.35 KDa
Source organism: Influenza A virus (A/canine/Colorado/17864/2006(H3N8))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: E0UVR5 (Residues: 17-344; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin (Ha1 Chain); Chain: A; domain 1
Hemagglutinin Chain: B
Molecule details ›
Chain: B
Length: 175 amino acids
Theoretical weight: 20.27 KDa
Source organism: Influenza A virus (A/canine/Colorado/17864/2006(H3N8))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: E0UVR5 (Residues: 345-516; Coverage: 30%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL, SIA, FUC
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P6322
Unit cell:
a: 96.926Å b: 96.926Å c: 349.576Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.235
Expression system: Spodoptera frugiperda

Quick links

Citations

9 review citations

Influenza virus reservoirs and intermediate hosts: dogs, horses, and new possibilities for influenza virus exposure of humans.
Parrish et al. (2015)
8 more