› 4uqb

X-ray diffraction
1.68Å resolution

X-ray structure of glucuronoxylan-xylanohydrolase (Xyn30A) from Clostridium thermocellum at 1.68 A resolution

Released:
DOI: 10.2210/pdb4uqb/pdb
PDB entry 4uqb coloured by chain, front view.
PDB entry 4uqb coloured by chain, side view.
PDB entry 4uqb coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
Conservation in the mechanism of glucuronoxylan hydrolysis revealed by the structure of glucuronoxylan xylanohydrolase (CtXyn30A) from Clostridium thermocellum.
Freire F, Verma A, Bule P, Alves VD, Fontes CM, Goyal A, Najmudin S
Acta Crystallogr D Struct Biol 72 1162-1173 (2016)
PMID: 27841749

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107089 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate binding family 6 Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 46.45 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A3DJS9 (Residues: 34-419; Coverage: 61%)
Gene name: Cthe_3012
Sequence domains: Glycosyl hydrolase family 30 beta sandwich domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P1
Unit cell:
a: 46.98Å b: 50.59Å c: 59.31Å
α: 114.77° β: 101.43° γ: 103.85°
R-values:
R R work R free
0.181 0.179 0.206
Expression system: Escherichia coli BL21

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Citations

4 citation in other articles

A novel fungal GH30 xylanase with xylobiohydrolase auxiliary activity.
Katsimpouras et al. (2019)
3 more