› 4us3

X-ray diffraction
2.1Å resolution

Crystal Structure of the bacterial NSS member MhsT in an Occluded Inward-Facing State

Released:
DOI: 10.2210/pdb4us3/pdb
PDB entry 4us3 coloured by chain, front view.
PDB entry 4us3 coloured by chain, side view.
PDB entry 4us3 coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Primary publication:
A mechanism for intracellular release of Na+ by neurotransmitter/sodium symporters.
Malinauskaite L, Quick M, Reinhard L, Lyons JA, Yano H, Javitch JA, Nissen P
Nat Struct Mol Biol 21 1006-12 (2014)
PMID: 25282149

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sodium-dependent transporter Chain: A
Molecule details ›
Chain: A
Length: 455 amino acids
Theoretical weight: 48.36 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Lactococcus lactis subsp. cremoris NZ9000
UniProt:
  • Canonical: Q9KDT3 (Residues: 2-453; Coverage: 100%)
Gene name: BH1128
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

4 bound ligands:
Ligand TRP 1 x TRP
Ligand NA 2 x NA
Ligand BNG 4 x BNG
Ligand LMU 6 x LMU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P2
Unit cell:
a: 44.28Å b: 49.89Å c: 110.05Å
α: 90° β: 96.76° γ: 90°
R-values:
R R work R free
0.192 0.191 0.234
Expression system: Lactococcus lactis subsp. cremoris NZ9000

Quick links

Citations

14 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
13 more

13 mentions without citation

Monoamine transporters: insights from molecular dynamics simulations.
Grouleff et al. (2015)
12 more