4usg

X-ray diffraction
1.97Å resolution

Crystal structure of PC4 W89Y mutant complex with DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-118213 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Activated RNA polymerase II transcriptional coactivator p15 Chains: A, B
Molecule details ›
Chains: A, B
Length: 66 amino acids
Theoretical weight: 7.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53999 (Residues: 63-127; Coverage: 51%)
Gene names: PC4, RPO2TC1, SUB1
Sequence domains: Transcriptional Coactivator p15 (PC4)
Structure domains: Transcriptional Coactivator Pc4; Chain A
5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP *TP*TP*TP*TP*TP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.06 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 67.66Å b: 67.66Å c: 120.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.212
Expression systems:
  • Escherichia coli
  • Not provided