› 4ut5

X-ray diffraction
1.75Å resolution

Crystal structure of the LecB lectin from Pseudomonas aeruginosa strain PA7 in complex with lewis a tetrasaccharide

Released:
DOI: 10.2210/pdb4ut5/pdb
PDB entry 4ut5 coloured by chain, front view.
PDB entry 4ut5 coloured by chain, side view.
PDB entry 4ut5 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PA7
Primary publication:
Genomic Rearrangements and Functional Diversification of lecA and lecB Lectin-Coding Regions Impacting the Efficacy of Glycomimetics Directed against Pseudomonas aeruginosa.
Boukerb AM, Decor A, Ribun S, Tabaroni R, Rousset A, Commin L, Buff S, Doléans-Jordheim A, Vidal S, Varrot A, Imberty A, Cournoyer B
Front Microbiol 7 811 (2016)
PMID: 27303392

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108127 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Calcium-mediated lectin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 114 amino acids
Theoretical weight: 11.66 KDa
Source organism: Pseudomonas aeruginosa PA7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6V267 (Residues: 2-115; Coverage: 99%)
Gene name: PSPA7_1769
Sequence domains: Fucose-binding lectin II (PA-IIL)
Structure domains: Calcium-mediated lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL, NAG, FUC, GLA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL, NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand CA 8 x CA
Ligand GAL 1 x GAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 52.79Å b: 70.37Å c: 54.66Å
α: 90° β: 90.5° γ: 90°
R-values:
R R work R free
0.149 0.148 0.183
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Pathoblockers or antivirulence drugs as a new option for the treatment of bacterial infections.
Calvert et al. (2018)
3 more

1 mention without citation

Genomic Rearrangements and Functional Diversification of lecA and lecB Lectin-Coding Regions Impacting the Efficacy of Glycomimetics Directed against Pseudomonas aeruginosa.
Boukerb et al. (2016)