› 4uts

X-ray diffraction
2.03Å resolution

Room temperature crystal structure of the fast switching M159T mutant of fluorescent protein Dronpa

Released:
DOI: 10.2210/pdb4uts/pdb
PDB entry 4uts coloured by chain, front view.
PDB entry 4uts coloured by chain, side view.
PDB entry 4uts coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
Room temperature crystal structure of the fast switching M159T mutant of the fluorescent protein dronpa.
Kaucikas M, Fitzpatrick A, Bryan E, Struve A, Henning R, Kosheleva I, Srajer V, Groenhof G, Van Thor JJ
Proteins 83 397-402 (2015)
PMID: 25524427

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 214 amino acids
Theoretical weight: 24.51 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli M15
UniProt:
  • Canonical: Q5TLG6 (Residues: 2-217; Coverage: 96%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYC 4 x GYC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 75.655Å b: 111.14Å c: 117.572Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.213
Expression system: Escherichia coli M15

Quick links

Citations

3 citation in other articles

Specific radiation damage is a lesser concern at room temperature.
Gotthard et al. (2019)
2 more

1 mention without citation

Room temperature crystal structure of the fast switching M159T mutant of the fluorescent protein dronpa.
Kaucikas et al. (2015)