› 4uu4

X-ray diffraction
2.75Å resolution

Crystal structure of LptH, the LptA homologous periplasmic component of the conserved lipopolysaccharide transport device from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb4uu4/pdb
PDB entry 4uu4 coloured by chain, front view.
PDB entry 4uu4 coloured by chain, side view.
PDB entry 4uu4 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Crystal structure of LptH, the periplasmic component of the lipopolysaccharide transport machinery from Pseudomonas aeruginosa.
Bollati M, Villa R, Gourlay LJ, Benedet M, Dehò G, Polissi A, Barbiroli A, Martorana AM, Sperandeo P, Bolognesi M, Nardini M
FEBS J 282 1980-97 (2015)
PMID: 25735820

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipopolysaccharide export system protein LptH Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.15 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HVV7 (Residues: 1-175; Coverage: 100%)
Gene names: PA4460, lptA, lptH
Sequence domains: LptA/(LptD N-terminal domain) LPS transport protein
Structure domains: Lipopolysaccharide (LPS) transport protein A like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 45Å b: 45Å c: 146.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.235 0.296
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The lipopolysaccharide transport (Lpt) machinery: A nonconventional transporter for lipopolysaccharide assembly at the outer membrane of Gram-negative bacteria.
Sperandeo et al. (2017)
6 more

3 mentions without citation

Crystal structure of LptH, the periplasmic component of the lipopolysaccharide transport machinery from Pseudomonas aeruginosa.
Bollati et al. (2015)
2 more