4uuj

X-ray diffraction
2.4Å resolution

POTASSIUM CHANNEL KCSA-FAB WITH TETRAHEXYLAMMONIUM

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-209479 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ANTIBODY FAB FRAGMENT LIGHT CHAIN Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
ANTIBODY FAB FRAGMENT HEAVY CHAIN Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 111 amino acids
Theoretical weight: 11.69 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 22-124; Coverage: 64%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I4
Unit cell:
a: 154.615Å b: 154.615Å c: 75.953Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.248
Expression systems:
  • Mus musculus
  • Escherichia coli