› 4uus

X-ray diffraction
2.55Å resolution

CRYSTAL STRUCTURE OF A UBX-EXD-DNA COMPLEX INCLUDING THE UBDA MOTIF

Released:
DOI: 10.2210/pdb4uus/pdb
PDB entry 4uus coloured by chain, front view.
PDB entry 4uus coloured by chain, side view.
PDB entry 4uus coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
A flexible extension of the Drosophila ultrabithorax homeodomain defines a novel Hox/PBC interaction mode.
Foos N, Maurel-Zaffran C, Maté MJ, Vincentelli R, Hainaut M, Berenger H, Pradel J, Saurin AJ, Ortiz-Lombardía M, Graba Y
Structure 23 270-9 (2015)
PMID: 25651060

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118215 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Homeotic protein ultrabithorax Chains: A, E
Molecule details ›
Chains: A, E
Length: 77 amino acids
Theoretical weight: 9.59 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83949 (Residues: 292-367; Coverage: 20%)
Gene names: CG10388, Ubx
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
Homeobox protein extradenticle Chains: B, F
Molecule details ›
Chains: B, F
Length: 76 amino acids
Theoretical weight: 8.86 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40427 (Residues: 238-312; Coverage: 20%)
Gene names: CG8933, exd
Sequence domains: Homeobox KN domain
Structure domains: Homeodomain-like
5'-D(*GP*TP*CP*GP*CP*CP*AP*TP*AP*AP*AP*TP*CP*AP*C)-3' Chains: C, G
Molecule details ›
Chains: C, G
Length: 15 nucleotides
Theoretical weight: 4.54 KDa
5'-D(*AP*CP*GP*TP*GP*AP*TP*TP*TP*AP*TP*GP*GP*CP*G)-3' Chains: D, H
Molecule details ›
Chains: D, H
Length: 15 nucleotides
Theoretical weight: 4.64 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 42.67Å b: 95.92Å c: 69.82Å
α: 90° β: 99.34° γ: 90°
R-values:
R R work R free
0.19 0.187 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Homeodomain proteins: an update.
Bürglin et al. (2016)
5 more

1 mention without citation

Structural Features of Transcription Factors Associating with Nucleosome Binding.
Fernandez Garcia et al. (2019)