› 4uuu

X-ray diffraction
1.71Å resolution

1.7 A resolution structure of human cystathionine beta-synthase regulatory domain (del 516-525) in complex with SAM

Released:
DOI: 10.2210/pdb4uuu/pdb
PDB entry 4uuu coloured by chain, front view.
PDB entry 4uuu coloured by chain, side view.
PDB entry 4uuu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inter-domain communication of human cystathionine β-synthase: structural basis of S-adenosyl-L-methionine activation.
McCorvie TJ, Kopec J, Hyung SJ, Fitzpatrick F, Feng X, Termine D, Strain-Damerell C, Vollmar M, Fleming J, Janz JM, Bulawa C, Yue WW
J Biol Chem 289 36018-30 (2014)
PMID: 25336647

Function and Biology Details

Reaction catalysed: 
L-serine + L-homocysteine = L-cystathionine + H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152934 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine beta-synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 155 amino acids
Theoretical weight: 17.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35520 (Residues: 406-547; Coverage: 24%)
Gene name: CBS
Sequence domains: CBS domain
Structure domains: CBS-domain

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
1 bound ligand:
Ligand EDO 12 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 39.85Å b: 78.65Å c: 90.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.199
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Cysteine metabolic circuitries: druggable targets in cancer.
Bonifácio et al. (2021)
2 more

2 mentions without citation

From structural biology to designing therapy for inborn errors of metabolism.
Yue WW. (2016)
1 more