› 4uv2

X-ray diffraction
2.8Å resolution

Structure of the curli transport lipoprotein CsgG in a non-lipidated, pre-pore conformation

Released:
DOI: 10.2210/pdb4uv2/pdb
PDB entry 4uv2 coloured by chain, front view.
PDB entry 4uv2 coloured by chain, side view.
PDB entry 4uv2 coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. MC4100
Primary publication:
Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG.
Goyal P, Krasteva PV, Van Gerven N, Gubellini F, Van den Broeck I, Troupiotis-Tsaïlaki A, Jonckheere W, Péhau-Arnaudet G, Pinkner JS, Chapman MR, Hultgren SJ, Howorka S, Fronzes R, Remaut H
Nature 516 250-3 (2014)
PMID: 25219853

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assemblies composition:
homo octamer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142425 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Curli production assembly/transport component CsgG Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 262 amino acids
Theoretical weight: 29.18 KDa
Source organism: Escherichia coli str. K-12 substr. MC4100
Expression system: Escherichia coli B
UniProt:
  • Canonical: P0AEA2 (Residues: 17-277; Coverage: 100%)
Gene names: JW1020, b1037, csgG
Sequence domains: Curli production assembly/transport component CsgG

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 96 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 101.33Å b: 103.6Å c: 141.74Å
α: 111.33° β: 90.55° γ: 118.21°
R-values:
R R work R free
0.19 0.188 0.234
Expression system: Escherichia coli B

Quick links

Citations

51 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
50 more

1 mention without citation

Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG.
Goyal et al. (2014)