› 4uvm

X-ray diffraction
3Å resolution

In meso crystal structure of the POT family transporter PepTSo

Released:
DOI: 10.2210/pdb4uvm/pdb
PDB entry 4uvm coloured by chain, front view.
PDB entry 4uvm coloured by chain, side view.
PDB entry 4uvm coloured by chain, top view.
Source organism: Shewanella oneidensis MR-1
Primary publication:
Gating topology of the proton-coupled oligopeptide symporters.
Fowler PW, Orwick-Rydmark M, Radestock S, Solcan N, Dijkman PM, Lyons JA, Kwok J, Caffrey M, Watts A, Forrest LR, Newstead S
Structure 23 290-301 (2015)
PMID: 25651061

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione uptake transporter Chain: A
Molecule details ›
Chain: A
Length: 524 amino acids
Theoretical weight: 57.68 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8EKT7 (Residues: 4-516; Coverage: 99%)
Gene name: SO_0002
Sequence domains: POT family
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

2 bound ligands:
Ligand 78M 1 x 78M
Ligand 78N 4 x 78N
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P41212
Unit cell:
a: 86.83Å b: 86.83Å c: 219.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
11 more

12 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
11 more