› 4ux5

X-ray diffraction
2.4Å resolution

Structure of DNA complex of PCG2

Released:
DOI: 10.2210/pdb4ux5/pdb
PDB entry 4ux5 coloured by chain, front view.
PDB entry 4ux5 coloured by chain, side view.
PDB entry 4ux5 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of DNA recognition by PCG2 reveals a novel DNA binding mode for winged helix-turn-helix domains.
Liu J, Huang J, Zhao Y, Liu H, Wang D, Yang J, Zhao W, Taylor IA, Peng YL
Nucleic Acids Res 43 1231-40 (2015)
PMID: 25550425

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236843 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH APSES-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 136 amino acids
Theoretical weight: 15.19 KDa
Source organism: Pyricularia oryzae
UniProt:
  • Canonical: G4NA99 (Residues: 1-138; Coverage: 19%)
Gene name: MGG_08463
Sequence domains: KilA-N domain
Structure domains: Transcription regulator HTH, APSES-type DNA-binding domain
5'-D(*CP*AP*AP*TP*GP*AP*CP*GP*CP*GP*TP*AP*AP*GP)-3' Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.31 KDa
Source organism: Candida albicans
Expression system: Not provided
5'-D(*CP*TP*TP*AP*CP*GP*CP*GP*TP*CP*AP*TP*TP*GP)-3' Chain: D
Molecule details ›
Chain: D
Length: 14 nucleotides
Theoretical weight: 4.25 KDa
Source organism: Candida albicans
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P4212
Unit cell:
a: 117.36Å b: 117.36Å c: 65.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.258
Expression system: Not provided

Quick links

Citations

1 review citation

The evolution of a G1/S transcriptional network in yeasts.
Hendler et al. (2018)
9 other articles

1 mention without citation

Structural basis of DNA recognition by PCG2 reveals a novel DNA binding mode for winged helix-turn-helix domains.
Liu et al. (2015)