› 4uxh

X-ray diffraction
2.4Å resolution

Leishmania major Thymidine Kinase in complex with AP5dT

Released:
DOI: 10.2210/pdb4uxh/pdb
PDB entry 4uxh coloured by chain, front view.
PDB entry 4uxh coloured by chain, side view.
PDB entry 4uxh coloured by chain, top view.
Source organism: Leishmania major
Primary publication:
Structural and Kinetic Characterization of Thymidine Kinase from Leishmania major.
Timm J, Bosch-Navarrete C, Recio E, Nettleship JE, Rada H, González-Pacanowska D, Wilson KS
PLoS Negl Trop Dis 9 e0003781 (2015)
PMID: 25978379

Function and Biology Details

Reaction catalysed: 
ATP + thymidine = ADP + thymidine 5'-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 21.03 KDa
Source organism: Leishmania major
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q4QC75 (Residues: 2-183; Coverage: 64%)
Gene name: LMJF_21_1210
Sequence domains: Thymidine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand T5A 2 x T5A
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P6522
Unit cell:
a: 64.149Å b: 64.149Å c: 324.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.195 0.257
Expression system: Spodoptera frugiperda

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Citations

4 review citations

Nuclear DNA replication and repair in parasites of the genus Leishmania: Exploiting differences to develop innovative therapeutic approaches.
Uzcanga et al. (2017)
3 more

1 mention without citation

Structural and Kinetic Characterization of Thymidine Kinase from Leishmania major.
Timm et al. (2015)