› 4uz9

X-ray diffraction
2.2Å resolution

STRUCTURE OF THE WNT DEACYLASE NOTUM - CRYSTAL FORM VII - SOS COMPLEX - 2.2A

Released:
DOI: 10.2210/pdb4uz9/pdb
PDB entry 4uz9 coloured by chain, front view.
PDB entry 4uz9 coloured by chain, side view.
PDB entry 4uz9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Notum deacylates Wnt proteins to suppress signalling activity.
Kakugawa S, Langton PF, Zebisch M, Howell S, Chang TH, Liu Y, Feizi T, Bineva G, O'Reilly N, Snijders AP, Jones EY, Vincent JP
Nature 519 187-192 (2015)
PMID: 25731175

Function and Biology Details

Reaction catalysed: 
[Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine + H(2)O = [Wnt]-L-serine + (9Z)-hexadec-9-enoate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Palmitoleoyl-protein carboxylesterase NOTUM Chain: A
Molecule details ›
Chain: A
Length: 383 amino acids
Theoretical weight: 43.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6P988 (Residues: 81-451; Coverage: 78%)
Gene names: NOTUM, OK/SW-CL.30
Sequence domains: Pectinacetylesterase

Ligands and Environments

Carbohydrate polymer : NEW Components: GU4, YYJ
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P41212
Unit cell:
a: 69.63Å b: 69.63Å c: 195.148Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.237
Expression system: Homo sapiens

Quick links

Citations

70 review citations

Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities.
Nusse et al. (2017)
69 more

2 mentions without citation

Notum deacylates Wnt proteins to suppress signalling activity.
Kakugawa et al. (2015)
1 more