› 4v06

X-ray diffraction
2.63Å resolution

Crystal structure of human tryptophan hydroxylase 2 (TPH2), catalytic domain

Released:
DOI: 10.2210/pdb4v06/pdb
PDB entry 4v06 coloured by chain, front view.
PDB entry 4v06 coloured by chain, side view.
PDB entry 4v06 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kopec J, Oberholzer A, Fitzpatrick F, Newman J, Tallant C, Kiyani W, Shrestha L, Burgess-Brown N, von Delft F, Arrowsmith C, Edwards A, Bountra C, Yue WW

Function and Biology Details

Reaction catalysed: 
L-tryptophan + tetrahydrobiopterin + O(2) = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184897 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan 5-hydroxylase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 40.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IWU9 (Residues: 148-490; Coverage: 70%)
Gene names: NTPH, TPH2
Sequence domains: Biopterin-dependent aromatic amino acid hydroxylase
Structure domains: Aromatic amino acid hydroxylase

Ligands and Environments

2 bound ligands:
Ligand IMD 2 x IMD
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21212
Unit cell:
a: 95.96Å b: 100.199Å c: 89.009Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.242
Expression system: Escherichia coli BL21(DE3)

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