› 4v12

X-ray diffraction
1.5Å resolution

Crystal structure of the MSMEG_6754 dehydratase from Mycobacterium smegmatis

Released:
DOI: 10.2210/pdb4v12/pdb
PDB entry 4v12 coloured by chain, front view.
PDB entry 4v12 coloured by chain, side view.
PDB entry 4v12 coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis
Primary publication:
A new dehydratase conferring innate resistance to thiacetazone and intra-amoebal survival of Mycobacterium smegmatis.
Carrère-Kremer S, Blaise M, Singh VK, Alibaud L, Tuaillon E, Halloum I, van de Weerd R, Guérardel Y, Drancourt M, Takiff H, Geurtsen J, Kremer L
Mol Microbiol 96 1085-102 (2015)
PMID: 25754266

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MaoC like domain protein Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 37.02 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0R724 (Residues: 1-342; Coverage: 100%)
Gene name: MSMEG_6754
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand POG 1 x POG
Ligand BTB 1 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P41212
Unit cell:
a: 84.39Å b: 84.39Å c: 122.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.178
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Mycolic acids: deciphering and targeting the Achilles' heel of the tubercle bacillus.
Nataraj et al. (2015)
2 more

1 mention without citation

Deletion of a dehydratase important for intracellular growth and cording renders rough Mycobacterium abscessus avirulent.
Halloum et al. (2016)