› 4v2i

X-ray diffraction
1.69Å resolution

Biochemical characterization and structural analysis of a new cold- active and salt tolerant esterase from the marine bacterium Thalassospira sp

Released:
DOI: 10.2210/pdb4v2i/pdb
PDB entry 4v2i coloured by chain, front view.
PDB entry 4v2i coloured by chain, side view.
PDB entry 4v2i coloured by chain, top view.
Source organism: Thalassospira sp. GB04J01
Primary publication:
Biochemical characterization and structural analysis of a new cold-active and salt-tolerant esterase from the marine bacterium Thalassospira sp.
De Santi C, Leiros HK, Di Scala A, de Pascale D, Altermark B, Willassen NP
Extremophiles 20 323-36 (2016)
PMID: 27016194

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 34.96 KDa
Source organism: Thalassospira sp. GB04J01
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A023T3X2 (Residues: 28-343; Coverage: 100%)
Gene name: Est2349
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 73.326Å b: 85.429Å c: 91.747Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.143 0.182
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Enzymes from Marine Polar Regions and Their Biotechnological Applications.
Bruno et al. (2019)
6 more

9 mentions without citation

Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes.
Santiago et al. (2016)
8 more