› 4v2p

X-ray diffraction
1.67Å resolution

Ketosynthase MxnB

Released:
DOI: 10.2210/pdb4v2p/pdb
PDB entry 4v2p coloured by chain, front view.
PDB entry 4v2p coloured by chain, side view.
PDB entry 4v2p coloured by chain, top view.
Source organism: Myxococcus fulvus
Primary publication:
In vitro reconstitution of α-pyrone ring formation in myxopyronin biosynthesis.
Sucipto H, Sahner JH, Prusov E, Wenzel SC, Hartmann RW, Koehnke J, Müller R
Chem Sci 6 5076-5085 (2015)
PMID: 29308173

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ketosynthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 351 amino acids
Theoretical weight: 38.85 KDa
Source organism: Myxococcus fulvus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: T1SF45 (Residues: 1-335; Coverage: 100%)
Gene name: mxnB
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

4 bound ligands:
Ligand PO4 7 x PO4
Ligand MPD 4 x MPD
Ligand CL 1 x CL
Ligand NA 2 x NA
1 modified residue:
Ligand CSD 2 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 50.19Å b: 58.79Å c: 62.82Å
α: 104.75° β: 105.48° γ: 93.9°
R-values:
R R work R free
0.147 0.146 0.177
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Biosynthesis of polyketides by trans-AT polyketide synthases.
Helfrich et al. (2016)
2 more

1 mention without citation

In vitro reconstitution of α-pyrone ring formation in myxopyronin biosynthesis.
Sucipto et al. (2015)