› 4v41

X-ray diffraction
2.5Å resolution

E. COLI (LAC Z) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-MONOCLINIC)

Released:
DOI: 10.2210/pdb4v41/pdb
PDB entry 4v41 coloured by chain, front view.
PDB entry 4v41 coloured by chain, side view.
PDB entry 4v41 coloured by chain, top view.
Source organism: Escherichia coli BL21
Primary publication:
High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation.
Juers DH, Jacobson RH, Wigley D, Zhang XJ, Huber RE, Tronrud DE, Matthews BW
Protein Sci 9 1685-99 (2000)
PMID: 11045615

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 1023 amino acids
Theoretical weight: 116.7 KDa
Source organism: Escherichia coli BL21
UniProt:
  • Canonical: P00722 (Residues: 2-1024; Coverage: 100%)
Gene names: JW0335, b0344, lacZ
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand MG 32 x MG
1 modified residue:
Ligand CME 48 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 107.9Å b: 207.5Å c: 509.9Å
α: 90° β: 94.7° γ: 90°
R-values:
R R work R free
0.199 0.199 0.207

Quick links

Citations

15 review citations

Role of Na+ and K+ in enzyme function.
Page et al. (2006)
14 more