› 4v45

X-ray diffraction
2.6Å resolution

E. COLI (lacZ) BETA-GALACTOSIDASE-TRAPPED 2-F-GALACTOSYL-ENZYME INTERMEDIATE

Released:
DOI: 10.2210/pdb4v45/pdb
PDB entry 4v45 coloured by chain, front view.
PDB entry 4v45 coloured by chain, side view.
PDB entry 4v45 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A structural view of the action of Escherichia coli (lacZ) beta-galactosidase.
Juers DH, Heightman TD, Vasella A, McCarter JD, Mackenzie L, Withers SG, Matthews BW
Biochemistry 40 14781-94 (2001)
PMID: 11732897

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 1023 amino acids
Theoretical weight: 116.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00722 (Residues: 2-1024; Coverage: 100%)
Gene names: JW0335, b0344, lacZ
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand 2FG 16 x 2FG
Ligand MG 32 x MG
Ligand NA 32 x NA
1 modified residue:
Ligand CME 48 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 107.5Å b: 207.2Å c: 510.2Å
α: 90° β: 95° γ: 90°
R-values:
R R work R free
0.23 not available not available
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Role of Na+ and K+ in enzyme function.
Page et al. (2006)
12 more